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Optical Engineering

Surface immobilization of biomolecules by light
Author(s): Hans Sigrist; Andre Collioud; Jean-Francois Clemence; Hui Gao; Reto Luginbuehl; Michael Saenger; Gajendran Sundarababu
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Paper Abstract

Biomolecules performing specific biological functions on material surfaces are progressively employed in the development of miniaturized bioassays, biosensors, bioelectronic devices, and medical equipment. Device performance is improved with covalently immobilized bioconstituents. The unique advantages of using light-controlled reactions to achieve biomolecule immobilization on surfaces are addressed. On activation of introduced light-sensitive reagents, biomolecules are covalently linked to material surfaces. Procedures leading to light-dependent engineering of surfaces are exceptionally facile. Immobilization by light is compatible with biological functions, enabling surface patterning and molecular coating of materials. Current strategies and protocols are illustrated with selected examples of biomolecule photoimmobilization.

Paper Details

Date Published: 1 August 1995
PDF: 10 pages
Opt. Eng. 34(8) doi: 10.1117/12.201815
Published in: Optical Engineering Volume 34, Issue 8
Show Author Affiliations
Hans Sigrist, Univ. of Bern (Switzerland)
Andre Collioud, Univ. of Bern (Switzerland)
Jean-Francois Clemence, Univ. of Bern (Switzerland)
Hui Gao, Univ. of Bern (Switzerland)
Reto Luginbuehl, Univ. of Bern (Switzerland)
Michael Saenger, Univ. of Bern (Switzerland)
Gajendran Sundarababu, Univ. of Bern (Switzerland)

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