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Optical Engineering

Construction and biomedical application of immunosensors based on fiber optics and enhanced chemiluminescence
Author(s): Nickolaj F. Starodub; Pavel Ya. Arenkov; Alexander N. Starodub; Vladimir A. Berezin
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Paper Abstract

The main scheme of the measuring cell as well as the sensor device, the analysis conditions, and the results of the quantitative determination of several antigens are presented. Special attention is paid to the immobilization of one of the immunocomponents on the fiber optic surface and three approaches were investigated: (1) covalent binding of the immunocomponent with the help of glutaraldehyde to the surface preliminary treated by aminopropilethoxysilane, (2) direct binding of the immunocomponent to silicon oxide surface by BrCN, and (3) usage of a special membrane closely connected to the fiber optics. The investigations showed that the level of immobilized biomolecules may be achieved almost two fold more by the second approach in comparison with the first one. Good results can also be received by the usage of the special membrane. The sensitivity of the fiber optic immunosensors intended for the determination of the antigens selected was comparable with that made by the enzyme-linked immunosorbent assay (ELISA) method. But in all cases the rate of analyses by immunosensors was much higher than that of the ELISA method, which is the. main advantage of such types of immunosensors.

Paper Details

Date Published: 1 September 1994
PDF: 6 pages
Opt. Eng. 33(9) doi: 10.1117/12.177506
Published in: Optical Engineering Volume 33, Issue 9
Show Author Affiliations
Nickolaj F. Starodub, Ukrainian SSR Academy of Sciences (Ukraine)
Pavel Ya. Arenkov, Ukranian Academy of Science (Ukraine)
Alexander N. Starodub, Ukrainian Academy of Sciences (Russia)
Vladimir A. Berezin, Dnipropetrovsk State Univ. (Ukraine)

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