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Optical Engineering

Artificial testing targets with controllable blur for adaptive optics microscopes
Author(s): Masayuki Hattori; Yosuke Tamada; Takashi Murata; Shin Oya; Mitsuyasu Hasebe; Yutaka Hayano; Yasuhiro Kamei
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Paper Abstract

This letter proposes a method of configuring a testing target to evaluate the performance of adaptive optics microscopes. In this method, a testing slide with fluorescent beads is used to simultaneously determine the point spread function and the field of view. The point spread function is reproduced to simulate actual biological samples by etching a microstructure on the cover glass. The fabrication process is simplified to facilitate an onsite preparation. The artificial tissue consists of solid materials and silicone oil and is stable for use in repetitive experiments.

Paper Details

Date Published: 28 August 2017
PDF: 4 pages
Opt. Eng. 56(8) 080502 doi: 10.1117/1.OE.56.8.080502
Published in: Optical Engineering Volume 56, Issue 8
Show Author Affiliations
Masayuki Hattori, National Institute for Basic Biology (Japan)
Yosuke Tamada, National Institute for Basic Biology (Japan)
Graduate Univ. for Advanced Studies (SOKENDAI) (Japan)
Takashi Murata, National Institute for Basic Biology (Japan)
Graduate Univ. for Advanced Studies (SOKENDAI) (Japan)
Shin Oya, Subaru Telescope, National Astronomical Observatory of Japan (Japan)
Mitsuyasu Hasebe, National Institute for Basic Biology (Japan)
Graduate University for Advanced Studies (SOKENDAI) (Japan)
Yutaka Hayano, Advanced Technology Ctr., NAOJ (Japan)
Graduate Univ. for Advanced Studies (SOKENDAI) (Japan)
Yasuhiro Kamei, National Institute for Basic Biology (Japan)
Graduate Univ. for Advanced Studies (SOKENDAI) (Japan)


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