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Optical Engineering

Fluorescent sensing with Fresnel microlenses for optofluidic systems
Author(s): Anna Siudzińska; Andrzej Miszczuk; Jacek Marczak; Katarzyna Komorowska
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Paper Abstract

The concept of fluorescent sensing in a microchannel equipped with focusing light Fresnel lenses has been demonstrated. The concept employs a line or array of Fresnel lenses generating a line or array of focused light spots within a microfluidic channel, to increase the sensitivity of fluorescent signal detection in the system. We have presented efficient methods of master mold fabrication based on the lithography method and focused ion beam milling. The flexible microchannel was fabricated by an imprint process with new thiolene-epoxy resin with a good ability to replicate even submicron-size features. For final imprinted lenses, the measured background to peak signal level shows more than nine times the increase in brightness at the center of the focal spot for the green part of the spectrum (532 nm). The effectiveness of the microlenses in fluorescent-marked Escherichia coli bacteria was confirmed in a basic fluoroscope experiment, showing the increase of the sensitivity of the detection by the order of magnitude.

Paper Details

Date Published: 22 May 2017
PDF: 8 pages
Opt. Eng. 56(5) 057106 doi: 10.1117/1.OE.56.5.057106
Published in: Optical Engineering Volume 56, Issue 5
Show Author Affiliations
Anna Siudzińska, Wroclaw Research Ctr. EIT+ (Poland)
Andrzej Miszczuk, Wroclaw Research Ctr. EIT+ (Poland)
Jacek Marczak, Wroclaw Research Ctr. EIT+ (Poland)
Katarzyna Komorowska, Wroclaw Research Ctr. EIT+ (Poland)


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