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Optical Engineering

Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells
Author(s): Jianglei Di; Yu Song; Teli Xi; Jiwei Zhang; Ying Li; Chaojie Ma; Kaiqiang Wang; Jianlin Zhao
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Paper Abstract

Biological cells are usually transparent with a small refractive index gradient. Digital holographic interferometry can be used in the measurement of biological cells. We propose a dual-wavelength common-path digital holographic microscopy for the quantitative phase imaging of biological cells. In the proposed configuration, a parallel glass plate is inserted in the light path to create the lateral shearing, and two lasers with different wavelengths are used as the light source to form the dual-wavelength composite digital hologram. The information of biological cells for different wavelengths is separated and extracted in the Fourier domain of the hologram, and then combined to a shorter wavelength in the measurement process. This method could improve the system’s temporal stability and reduce speckle noises simultaneously. Mouse osteoblastic cells and peony pollens are measured to show the feasibility of this method.

Paper Details

Date Published: 11 July 2017
PDF: 7 pages
Opt. Eng. 56(11) 111712 doi: 10.1117/1.OE.56.11.111712
Published in: Optical Engineering Volume 56, Issue 11
Show Author Affiliations
Jianglei Di, Northwestern Polytechnical Univ. (China)
Yu Song, Northwestern Polytechnical Univ. (China)
Teli Xi, Northwestern Polytechnical Univ. (China)
Jiwei Zhang, Northwestern Polytechnical Univ. (China)
Ying Li, Northwestern Polytechnical Univ. (China)
Chaojie Ma, Northwestern Polytechnical Univ. (China)
Kaiqiang Wang, Northwestern Polytechnical Univ. (China)
Jianlin Zhao, Northwestern Polytechnical Univ. (China)

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