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Optical Engineering

Active layer identification of photonic crystal waveguide biosensor chip for the detection of Escherichia coli
Author(s): Balveer Painam; Rajinder S. Kaler; Mukesh Kumar
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Paper Abstract

This work represents experimental and simulation analysis of photonic crystal waveguide (PCW)-based biosensor structures, which is used for detection of the Escherichia coli (E. coli) cell. A method is adopted for E. coli culture to measure length, diameter, and refractive index to finalize the structural design and to verify the suitability of PCW as a biosensor. This method is tested using DH5α strains of E. coli. The typical precisions of measurements are varied in ranges from 1.132 to 1.825  μm and from 0.447 to 0.66  μm for pathogen’s length and diameter, respectively. The measured distribution of samples over length and diameter are in correlation with the measurements performed by scanning electron microscope. After obtaining average length and diameter of cylindrical shaped E. coli cell, we consider these values for simulation analysis of designed PCW biosensor. E. coli cell is trapped in the middle of the PCW biosensor having three different types of waveguides, i.e., gallium arsenide/silicon dioxide (GaAs/SiO2), silicon/silicon dioxide (Si/SiO2), or silicon nitride/silicon dioxide (Si3N4/SiO2) to observe the maximum resonance shift and sensitivity. It is observed from the simulation data analysis that GaAs/SiO2 is the preferred PCW biosensor for the identification of E. coli.

Paper Details

Date Published: 28 July 2016
PDF: 4 pages
Opt. Eng. 55(7) 077105 doi: 10.1117/1.OE.55.7.077105
Published in: Optical Engineering Volume 55, Issue 7
Show Author Affiliations
Balveer Painam, Thapar Univ. (India)
Rajinder S. Kaler, Thapar Univ. (India)
Mukesh Kumar, Indian Institute of Technology Roorkee (India)

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