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Optical Engineering

Tunable narrowband volume holographic imaging spectrometer for macroscopic fluorescence molecular tomography
Author(s): Yanlu Lv; Jiulou Zhang; Dong Zhang; Lin Zhang; Jianwen Luo
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Paper Abstract

A simple, compact, and inexpensive tunable volume holographic imaging spectrometer is presented to acquire narrowband multispectral fluorescence measurements. It is constructed with three simple achromatic lenses, a transmission volume hologram, and a detector array. Experiments are carried out to evaluate the performance of this spectrometer. The experimental results indicate that the tunable spectrometer can operate at a wavelength range from 400 to 700 nm. Additionally, this spectrometer can provide a lateral field-of-view greater than 33 mm and a spatial resolution of 1.0 mm under narrow band illumination (full-width-half-maximum bandwidth 20 nm). Multispectral fluorescence molecular tomography experiments are conducted with this spectrometer to test the applicability of narrowband multispectral macroscopy.

Paper Details

Date Published: 23 December 2016
PDF: 9 pages
Opt. Eng. 55(12) 123113 doi: 10.1117/1.OE.55.12.123113
Published in: Optical Engineering Volume 55, Issue 12
Show Author Affiliations
Yanlu Lv, Tsinghua Univ. (China)
Jiulou Zhang, Tsinghua Univ. (China)
Dong Zhang, Tsinghua Univ. (China)
Lin Zhang, Tsinghua Univ. (China)
Jianwen Luo, Tsinghua Univ. (China)


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