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Optical Engineering

Object field expansion in spatial light modulator-based phase contrast microscopy
Author(s): Malte Hasler; Janek Stahl; Tobias Haist; Wolfgang Osten
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Paper Abstract

In order to expand the field of view in spatial light modulator-based phase contrast microscopy, we employ a two-image recording method with digital postprocessing. Using this approach, we suppress the superimposed unwanted diffraction orders on the image sensor, thus eliminating the necessity to assure a strict separation of the diffraction orders. These methods allow for a significantly smaller microscope setup and an increased effective spatial bandwidth product. We apply this method for a number of phase contrast methods to show its capabilities and limitations. Mainly, the signal-to-noise ratio is decreased by η/2. Furthermore, we employ a simple field-dependent aberration correction scheme to improve the image quality. √In order to expand the field of view in spatial light modulator-based phase contrast microscopy, we employ a two-image recording method with digital postprocessing. Using this approach, we suppress the superimposed unwanted diffraction orders on the image sensor, thus eliminating the necessity to assure a strict separation of the diffraction orders. These methods allow for a significantly smaller microscope setup and an increased effective spatial bandwidth product. We apply this method for a number of phase contrast methods to show its capabilities and limitations. Mainly, the signal-to-noise ratio is decreased by η/√2. Furthermore, we employ a simple field-dependent aberration correction scheme to improve the image quality.

Paper Details

Date Published: 20 April 2015
PDF: 6 pages
Opt. Eng. 54(4) 043107 doi: 10.1117/1.OE.54.4.043107
Published in: Optical Engineering Volume 54, Issue 4
Show Author Affiliations
Malte Hasler, Univ. Stuttgart (Germany)
Janek Stahl, Univ. Stuttgart (Germany)
Tobias Haist, Institut für Technische Optik (Germany)
Wolfgang Osten, Institut für Technische Optik (Germany)


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