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Optical Engineering

Detection and discrimination of microorganisms on various substrates with quantum cascade laser spectroscopy
Author(s): Amira C. Padilla-Jimenez; William Ortiz-Rivera; Carlos Rios-Velazquez; Iris Vazquez-Ayala; Samuel P. Hernández-Rivera
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Paper Abstract

Investigations focusing on devising rapid and accurate methods for developing signatures for microorganisms that could be used as biological warfare agents’ detection, identification, and discrimination have recently increased significantly. Quantum cascade laser (QCL)-based spectroscopic systems have revolutionized many areas of defense and security including this area of research. In this contribution, infrared spectroscopy detection based on QCL was used to obtain the mid-infrared (MIR) spectral signatures of Bacillus thuringiensis, Escherichia coli, and Staphylococcus epidermidis. These bacteria were used as microorganisms that simulate biothreats (biosimulants) very truthfully. The experiments were conducted in reflection mode with biosimulants deposited on various substrates including cardboard, glass, travel bags, wood, and stainless steel. Chemometrics multivariate statistical routines, such as principal component analysis regression and partial least squares coupled to discriminant analysis, were used to analyze the MIR spectra. Overall, the investigated infrared vibrational techniques were useful for detecting target microorganisms on the studied substrates, and the multivariate data analysis techniques proved to be very efficient for classifying the bacteria and discriminating them in the presence of highly IR-interfering media.

Paper Details

Date Published: 5 February 2014
PDF: 11 pages
Opt. Eng. 53(6) 061611 doi: 10.1117/1.OE.53.6.061611
Published in: Optical Engineering Volume 53, Issue 6
Show Author Affiliations
Amira C. Padilla-Jimenez, Univ. de Puerto Rico Mayagüez (United States)
William Ortiz-Rivera, Univ. de Puerto Rico Mayagüez (United States)
Carlos Rios-Velazquez, Univ. de Puerto Rico Mayagüez (United States)
Iris Vazquez-Ayala, Univ. de Puerto Rico Mayagüez (United States)
Samuel P. Hernández-Rivera, Univ. de Puerto Rico Mayagüez (United States)

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