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Optical Engineering

Time-gated stimulated emission depletion nanoscopy
Author(s): Yifan Wang; Cuifang Kuang; Zhaotai Gu; Yingke Xu; Shuai Li; Xiang Hao; Xu Liu
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Paper Abstract

We have designed and built a time-gated continuous wave stimulated emission depletion (CW-STED) nanoscopy to visualize microstructures beyond the diffraction limit. An off-line time-gating detection was performed with the help of time-correlated single-photon counting technique. Experimental results showed that before time-gating, the resolution of our system was about 75 nm with a depletion beam (592 nm) power of 200 mW. By using the off-line time-gating detection, the resolution was further improved to 38 nm. Biological samples were also used to test the performance of our time-gated CW-STED, and a resolution of 70 nm was achieved with a depletion beam (592 nm) power of 85 mW. Detailed principles of time-gated CW-STED were discussed in the text. The time-gated STED provides a better resolution with finite laser power.

Paper Details

Date Published: 25 September 2013
PDF: 9 pages
Opt. Eng. 52(9) 093107 doi: 10.1117/1.OE.52.9.093107
Published in: Optical Engineering Volume 52, Issue 9
Show Author Affiliations
Yifan Wang, Zhejiang Univ. (China)
Cuifang Kuang, Zhejiang Univ. (China)
Zhaotai Gu, Zhejiang Univ. (China)
Yingke Xu, Zhejiang Univ. (China)
Shuai Li, Zhejiang Univ. (China)
Xiang Hao, Zhejiang Univ. (China)
Xu Liu, Zhejiang Univ. (China)


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