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Optical Engineering

Dual-wavelength digital holographic imaging with phase background subtraction
Author(s): Alexander T. Khmaladze; Rebecca L. Matz; Joshua Jasensky; Emily Seeley; Mark M. Banaszak Holl; Zhan Chen
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Paper Abstract

Three-dimensional digital holographic microscopic phase imaging of objects that are thicker than the wavelength of the imaging light is ambiguous and results in phase wrapping. In recent years, several unwrapping methods that employed two or more wavelengths were introduced. These methods compare the phase information obtained from each of the wavelengths and extend the range of unambiguous height measurements. A straightforward dual-wavelength phase imaging method is presented which allows for a flexible tradeoff between the maximum height of the sample and the amount of noise the method can tolerate. For highly accurate phase measurements, phase unwrapping of objects with heights higher than the beat (synthetic) wavelength (i.e. the product of the original two wavelengths divided by their difference), can be achieved. Consequently, three-dimensional measurements of a wide variety of biological systems and microstructures become technically feasible. Additionally, an effective method of removing phase background curvature based on slowly varying polynomial fitting is proposed. This method allows accurate volume measurements of several small objects with the same image frame.

Paper Details

Date Published: 18 May 2012
PDF: 9 pages
Opt. Eng. 51(5) 055801 doi: 10.1117/1.OE.51.5.055801
Published in: Optical Engineering Volume 51, Issue 5
Show Author Affiliations
Alexander T. Khmaladze, Univ. of Michigan (United States)
Rebecca L. Matz, Univ. of Michigan (United States)
Joshua Jasensky, Univ. of Michigan (United States)
Emily Seeley, Univ. of Michigan (United States)
Mark M. Banaszak Holl, Univ. of Michigan (United States)
Zhan Chen, Univ. of Michigan (United States)

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