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Optical Engineering

Vertical differential interference contrast
Author(s): Michael Warber; Tobias Haist; Malte Hasler; Wolfgang Osten
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Paper Abstract

We propose a new phase contrast filtering technique based on a combination of a focused and a defocused point-spread-function. This way, an axial shear is introduced in the imaging system. Compared to conventional differential interference contrast, an isotropic behavior is achieved. The lateral resolution is improved compared to conventional defocusing. Furthermore, the digital combination of multiple images leads to strongly enhanced visualization of small structures. We show simulated results as well as experimental results using a spatial-light modulator-based microscope.

Paper Details

Date Published: 6 February 2012
PDF: 8 pages
Opt. Eng. 51(1) 013204 doi: 10.1117/1.OE.51.1.013204
Published in: Optical Engineering Volume 51, Issue 1
Show Author Affiliations
Michael Warber, Univ. Stuttgart (Germany)
Tobias Haist, Univ. Stuttgart (Germany)
Malte Hasler, Univ. Stuttgart (Germany)
Wolfgang Osten, Univ. Stuttgart (Germany)

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