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Neurophotonics • Open Access

Utah optrode array customization using stereotactic brain atlases and 3-D CAD modeling for optogenetic neocortical interrogation in small rodents and nonhuman primates
Author(s): Ronald W. Boutte; Sam Merlin; Guy Yona; Brandon Griffiths; Alessandra Angelucci; Itamar Kahn; Shy Shoham; Steve Blair

Paper Abstract

As the optogenetic field expands, the need for precise targeting of neocortical circuits only grows more crucial. This work demonstrates a technique for using Solidworks® computer-aided design (CAD) and readily available stereotactic brain atlases to create a three-dimensional (3-D) model of the dorsal region of area visual cortex 4 (V4D) of the macaque monkey (Macaca fascicularis) visual cortex. The 3-D CAD model of the brain was used to customize an 8×6 Utah optrode array (UOA) after it was determined that a high-density (13×13) UOA caused extensive damage to marmoset (Callithrix jacchus) primary visual cortex as assessed by electrophysiological recording of spiking activity through a 1.5-mm-diameter through glass via. The 8×6 UOA was customized for optrode length (400  μm), optrode width (≤100  μm), optrode pitch (400  μm), backplane thickness (500  μm), and overall form factor (3.45  mm×2.65  mm). Two 8×6 UOAs were inserted into layer VI of macaque V4D cortices with minimal damage as assessed in fixed tissue cytochrome oxidase staining in nonrecoverable surgeries. Additionally, two 8×6 arrays were implanted in mice (Mus musculus) motor cortices, providing early evidence for long-term tolerability (over 6 months), and for the ability to integrate the UOA with a Holobundle light delivery system toward patterned optogenetic stimulation of cortical networks.

Paper Details

Date Published: 12 July 2017
PDF: 9 pages
Neurophoton. 4(4) 041502 doi: 10.1117/1.NPh.4.4.041502
Published in: Neurophotonics Volume 4, Issue 4
Show Author Affiliations
Ronald W. Boutte, The Univ. of Utah (United States)
Northrop Grumman Corp. (United States)
Sam Merlin, The Univ. of Utah (United States)
Guy Yona, Technion–Israel Institute of Technology (Israel)
Brandon Griffiths, Northrop Grumman Corp. (United States)
Alessandra Angelucci, The Univ. of Utah (United States)
Itamar Kahn, Technion-Israel Institute of Technology (Israel)
Shy Shoham, Technion-Israel Institute of Technology (Israel)
Steve Blair, The Univ. of Utah (United States)

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