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Neurophotonics

Imaging membrane potential changes from dendritic spines using computer-generated holography
Author(s): Dimitrii Tanese; Ju-Yun Weng; Valeria Zampini; Vincent de-Sars; Marco Canepari; Balazs J. Rozsa; Valentina Emiliani; Dejan Zecevic
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Paper Abstract

Electrical properties of neuronal processes are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor electrical subthreshold events as they travel from synapses on distal dendrites and summate at particular locations to initiate action potentials. It is now possible to carry out these measurements at the scale of individual dendritic spines using voltage imaging. In these measurements, the voltage-sensitive probes can be thought of as transmembrane voltmeters with a linear scale, which directly monitor electrical signals. Grinvald et al. were important early contributors to the methodology of voltage imaging, and they pioneered some of its significant results. We combined voltage imaging and glutamate uncaging using computer-generated holography. The results demonstrated that patterned illumination, by reducing the surface area of illuminated membrane, reduces photodynamic damage. Additionally, region-specific illumination practically eliminated the contamination of optical signals from individual spines by the scattered light from the parent dendrite. Finally, patterned illumination allowed one-photon uncaging of glutamate on multiple spines to be carried out in parallel with voltage imaging from the parent dendrite and neighboring spines.

Paper Details

Date Published: 12 May 2017
PDF: 11 pages
Neurophoton. 4(3) 031211 doi: 10.1117/1.NPh.4.3.031211
Published in: Neurophotonics Volume 4, Issue 3
Show Author Affiliations
Dimitrii Tanese, Univ. Paris Descartes (France)
Ju-Yun Weng, Yale School of Medicine (United States)
Valeria Zampini, Univ. Paris Descartes (France)
Vincent de-Sars, Univ. Paris Descartes (France)
Marco Canepari, Univ. Grenoble Alpes - CNRS (France)
Lab. of Excellence in Ion Channel Science and Therapeutics (France)
Institut National de la Santé et de la Recherche Médicale (France)
Balazs J. Rozsa, Institute of Experimental Medicine, Hungarian Academy of Sciences (Hungary)
Valentina Emiliani, Univ. Paris Descartes (France)
Dejan Zecevic, Yale School of Medicine (United States)


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