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Neurophotonics

Ryanodine and IP3 receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation
Author(s): Gleb P. Tolstykh; Cory A. Olsovsky; Bennett L. Ibey; Hope T. Beier
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Paper Abstract

Pulsed infrared (IR) laser energy has been shown to modulate neurological activity through both stimulation and inhibition of action potentials. While the mechanism(s) behind this phenomenon is (are) not completely understood, certain hypotheses suggest that the rise in temperature from IR exposure could activate temperature- or pressure-sensitive ion channels or create pores in the cellular outer membrane, allowing an influx of typically plasma-membrane-impermeant ions. Studies using fluorescent intensity-based calcium ion ( Ca 2 + ) sensitive dyes show changes in Ca 2 + levels after various IR stimulation parameters, which suggests that Ca 2 + may originate from the external solution. However, activation of intracellular signaling pathways has also been demonstrated, indicating a more complex mechanism of increasing intracellular Ca 2 + concentration. We quantified the Ca 2 + mobilization in terms of influx from the external solution and efflux from intracellular organelles using Fura-2 and a high-speed ratiometric imaging system that rapidly alternates the dye excitation wavelengths. Using nonexcitable Chinese hamster ovarian ( CHO - hM 1 ) cells and neuroblastoma-glioma (NG108) cells, we demonstrate that intracellular IP 3 receptors play an important role in the IR-induced Ca 2 + , with the
Paper Details

Date Published: 5 April 2017
PDF: 8 pages
4(2) 025001 doi: 10.1117/1.NPh.4.2.025001
Published in: Neurophotonics Volume 4, Issue 2
Show Author Affiliations
Gleb P. Tolstykh, General Dynamics Information Technology (United States)
Cory A. Olsovsky, Texas A&M Univ. (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)
Hope T. Beier, Air Force Research Lab. (United States)


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