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Neurophotonics • Open Access • new

Imaging extracellular potassium dynamics in brain tissue using a potassium-sensitive nanosensor
Author(s): Joel Wellbourne-Wood; Theresa S. Rimmele; Jean-Yves Chatton

Paper Abstract

Neuronal activity results in the release of K + into the extracellular space (ECS). Classically, measurements of extracellular K + ( [ K + ] o ) are carried out using K + -sensitive microelectrodes, which provide a single point measurement with undefined spatial resolution. An imaging approach would enable the spatiotemporal mapping of [ K + ] o . Here, we report on the design and characterization of a fluorescence imaging-based K + -sensitive nanosensor for the ECS based on dendrimer nanotechnology. Spectral characterization, sensitivity, and selectivity of the nanosensor were assessed by spectrofluorimetry, as well as in both wide-field and two-photon microscopy settings, demonstrating the nanosensor efficacy over the physiologically relevant ion concentration range. Spatial and temporal kinetics of the nanosensor responses were assessed using a localized iontophoretic K + application on a two-photon imaging setup. Using acute mouse brain slices, we demonstrate that the nanosensor is retained in the ECS for extended periods of time. In addition, we present a ratiometric ve

Paper Details

Date Published: 9 February 2017
PDF: 9 pages
4(1) 015002 doi: 10.1117/1.NPh.4.1.015002
Published in: Neurophotonics Volume 4, Issue 1
Show Author Affiliations
Joel Wellbourne-Wood, Univ. de Lausanne (Switzerland)
Theresa S. Rimmele, Univ. de Lausanne (Switzerland)
Jean-Yves Chatton, Univ. de Lausanne (Switzerland)

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