Share Email Print
cover

Neurophotonics

Nanoscale organization of synaptic adhesion proteins revealed by single-molecule localization microscopy
Author(s): Ingrid Chamma; Florian Levet; Jean-Baptiste Sibarita; Matthieu Sainlos; Olivier Thoumine
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

The advent of superresolution imaging has created a strong need for both optimized labeling strategies and analysis methods to probe the nanoscale organization of complex biological structures. We present a thorough description of the distribution of synaptic adhesion proteins at the nanoscopic scale, namely presynaptic neurexin-1β (Nrx1β), and its two postsynaptic binding partners neuroligin-1 (Nlg1) and leucine-rich-repeat transmembrane protein 2 (LRRTM2). We monitored these proteins in the membrane of neurons by direct stochastic optical reconstruction microscopy, after live surface labeling with Alexa647-conjugated monomeric streptavidin. The small probe (∼3  nm) efficiently penetrates into crowded synaptic junctions and reduces the distance to target. We quantified the organization of the single-molecule localization data using a tesselation-based analysis technique. We show that Nlg1 exhibits a fairly disperse organization within dendritic spines, while LRRTM2 is organized in compact domains, and Nrx1β in presynaptic terminals displays a dual-organization pattern intermediate between that of Nlg1 and LRRTM2. These results suggest that part of Nrx1β interacts transsynaptically with Nlg1 and the other part with LRRTM2

Paper Details

Date Published: 3 November 2016
PDF: 7 pages
Neurophoton. 3(4) 041810 doi: 10.1117/1.NPh.3.4.041810
Published in: Neurophotonics Volume 3, Issue 4
Show Author Affiliations
Ingrid Chamma, Interdisciplinary Institute for NeuroScience (France)
Univ. Bordeaux Segalen (France)
Ctr. National de la Recherche Scientifique (France)
Florian Levet, Interdisciplinary Institute for NeuroScience (France)
Univ. Bordeaux Segalen (France)
Ctr. National de la Recherche Scientifique (France)
Jean-Baptiste Sibarita, Interdisciplinary Institute for NeuroScience (France)
Univ. Bordeaux Segalen (France)
Ctr. National de la Recherche Scientifique (France)
Matthieu Sainlos, Interdisciplinary Institute for NeuroScience (France)
Univ. Bordeaux Segalen (France)
Ctr. National de la Recherche Scientifique (France)
Olivier Thoumine, Interdisciplinary Institute for NeuroScience (France)
Univ. Bordeaux Segalen (France)
Ctr. National de la Recherche Scientifique (France)


© SPIE. Terms of Use
Back to Top