Share Email Print

Neurophotonics • Open Access

Filling the gap: adding super-resolution to array tomography for correlated ultrastructural and molecular identification of electrical synapses at the C. elegans connectome
Author(s): Sebastian Matthias Markert; Sebastian Britz; Sven Proppert; Marietta Lang; Daniel Witvliet; Ben Mulcahy; Markus Sauer; Mei Zhen; Jean-Louis Bessereau; Christian Stigloher

Paper Abstract

Correlating molecular labeling at the ultrastructural level with high confidence remains challenging. Array tomography (AT) allows for a combination of fluorescence and electron microscopy (EM) to visualize subcellular protein localization on serial EM sections. Here, we describe an application for AT that combines near-native tissue preservation via high-pressure freezing and freeze substitution with super-resolution light microscopy and high-resolution scanning electron microscopy (SEM) analysis on the same section. We established protocols that combine SEM with structured illumination microscopy (SIM) and direct stochastic optical reconstruction microscopy (

Paper Details

Date Published: 4 May 2016
PDF: 10 pages
Neurophoton. 3(4) 041802 doi: 10.1117/1.NPh.3.4.041802
Published in: Neurophotonics Volume 3, Issue 4
Show Author Affiliations
Sebastian Matthias Markert, Julius-Maximilians-Univ. Würzburg (Germany)
Sebastian Britz, Julius-Maximilians-Univ. Würzburg (Germany)
Sven Proppert, Julius-Maximilians-Univ. Würzburg (Germany)
University of Würzburg (Germany)
Marietta Lang, Julius-Maximilians-Univ. Würzburg (Germany)
Daniel Witvliet, Lunenfeld-Tanenbaum Research Institute (Canada)
Univ. of Toronto (Canada)
Ben Mulcahy, Lunenfeld-Tanenbaum Research Institute (Canada)
Univ. of Toronto (Canada)
Markus Sauer, Julius-Maximilians-Univ. Würzburg (Germany)
Mei Zhen, Lunenfeld-Tanenbaum Research Institute (Canada)
Univ. of Toronto (Canada)
Jean-Louis Bessereau, Univ. Claude Bernard Lyon 1 (France)
Christian Stigloher, Julius-Maximilians-Univ. Würzburg (Germany)

© SPIE. Terms of Use
Back to Top