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Neurophotonics • Open Access

Acute insertion effects of penetrating cortical microelectrodes imaged with quantitative optical coherence angiography
Author(s): Daniel X. Hammer; Andrea Lozzi; Adam Boretsky; Cristin G. Welle

Paper Abstract

The vascular response during cortical microelectrode insertion was measured with amplitude decorrelation-based quantitative optical coherence angiography (OCA). Four different shank-style microelectrode configurations were inserted in murine motor cortex beneath a surgically implanted window in discrete steps while OCA images were collected and processed for angiography and flowmetry. Quantitative measurements included tissue displacement (measured by optical flow), perfused capillary density, and capillary flow velocity. The primary effect of insertion was mechanical perturbation, the effects of which included tissue displacement, arteriolar rupture, and compression of a branch of the anterior cerebral artery causing a global decrease in flow. Other effects observed included local flow drop-out in the region immediately surrounding the microelectrode. The mean basal capillary network velocity for all animals was 0.23 (±0.05  SD) and 0.18 (±0.07  SD) mm/s for capillaries from 100 to 300  μm and 300 to 500  μm, respectively. Upon insertion, the 2-shank electrode arrays caused a decrease in capillary flow density and velocity, while the results from other configurations were not different from controls. The proximity to large vessels appears to play a larger role than the array configuration. These results can guide neurosurgeons and electrode designers to minimize trauma and ischemia during microelectrode insertion.

Paper Details

Date Published: 19 April 2016
PDF: 17 pages
Neurophoton. 3(2) 025002 doi: 10.1117/1.NPh.3.2.025002
Published in: Neurophotonics Volume 3, Issue 2
Show Author Affiliations
Daniel X. Hammer, U.S. Food and Drug Administration (United States)
Andrea Lozzi, U.S. Food and Drug Administration (United States)
Adam Boretsky, U.S. Food and Drug Administration (United States)
Cristin G. Welle, U.S. Food and Drug Administration (United States)
Univ. of Colorado Denver (United States)

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