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Neurophotonics • Open Access

Bioluminescence imaging in live cells and animals
Author(s): Jack K. Tung; Ken Berglund; Claire-Anne Gutekunst; Ute Hochgeschwender; Robert E. Gross

Paper Abstract

The use of bioluminescent reporters in neuroscience research continues to grow at a rapid pace as their applications and unique advantages over conventional fluorescent reporters become more appreciated. Here, we describe practical methods and principles for detecting and imaging bioluminescence from live cells and animals. We systematically tested various components of our conventional fluorescence microscope to optimize it for long-term bioluminescence imaging. High-resolution bioluminescence images from live neurons were obtained with our microscope setup, which could be continuously captured for several hours with no signs of phototoxicity. Bioluminescence from the mouse brain was also imaged noninvasively through the intact skull with a conventional luminescence imager. These methods demonstrate how bioluminescence can be routinely detected and measured from live cells and animals in a cost-effective way with common reagents and equipment.

Paper Details

Date Published: 5 April 2016
PDF: 6 pages
Neurophoton. 3(2) 025001 doi: 10.1117/1.NPh.3.2.025001
Published in: Neurophotonics Volume 3, Issue 2
Show Author Affiliations
Jack K. Tung, Georgia Institute of Technology (United States)
Emory Univ. (United States)
Ken Berglund, Emory Univ. (United States)
Claire-Anne Gutekunst, Emory Univ. (United States)
Ute Hochgeschwender, Central Michigan Univ. (United States)
Robert E. Gross, Emory Univ. (United States)
Georgia Institute of Technology (United States)

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