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Transcranial low-level laser therapy (810 nm) temporarily inhibits peripheral nociception: photoneuromodulation of glutamate receptors, prostatic acid phophatase, and adenosine triphosphate
Author(s): Marcelo Victor Pires de Sousa; Cleber Ferraresi; Masayoshi Kawakubo; Beatriz Kaippert; Elisabeth Mateus Yoshimura; Michael R. Hamblin
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Paper Abstract

Photobiomodulation or low-level light therapy has been shown to attenuate both acute and chronic pain, but the mechanism of action is not well understood. In most cases, the light is applied to the painful area, but in the present study we applied light to the head. We found that transcranial laser therapy (TLT) applied to mouse head with specific parameters (810 nm laser, 300  mW/cm2, 7.2 or 36  J/cm2) decreased the reaction to pain in the foot evoked either by pressure (von Frey filaments), cold, or inflammation (formalin injection) or in the tail (evoked by heat). The pain threshold increasing is maximum around 2 h after TLT, remains up to 6 h, and is finished 24 h after TLT. The mechanisms were investigated by quantification of adenosine triphosphate (ATP), immunofluorescence, and hematoxylin and eosin (H&E) staining of brain tissues. TLT increased ATP and prostatic acid phosphatase (an endogenous analgesic) and reduced the amount of glutamate receptor (mediating a neurotransmitter responsible for conducting nociceptive information). There was no change in the concentration of tubulin, a constituent of the cytoskeleton, and the H&E staining revealed no tissue damage.

Paper Details

Date Published: 25 January 2016
PDF: 9 pages
Neurophoton. 3(1) 015003 doi: 10.1117/1.NPh.3.1.015003
Published in: Neurophotonics Volume 3, Issue 1
Show Author Affiliations
Marcelo Victor Pires de Sousa, Massachusetts General Hospital (United States)
Univ. de São Paulo (Brazil)
Bright Photomedicine Ltd. (Brazil)
Cleber Ferraresi, Massachusetts General Hospital (United States)
Univ. Federal de São Carlos (Brazil)
Masayoshi Kawakubo, Wellman Ctr. for Photomedicine (United States)
Beatriz Kaippert, Wellman Ctr. for Photomedicine (United States)
Univ. Federal of Rio de Janeiro (Brazil)
Elisabeth Mateus Yoshimura, Univ. de São Paulo (Brazil)
Michael R. Hamblin, Wellman Ctr. for Photomedicine (United States)
Harvard Medical School (United States)
Massachusetts Institute of Technology (United States)

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