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Neurophotonics

Differential pathlength factor informs evoked stimulus response in a mouse model of Alzheimer’s disease
Author(s): Alexander J. Lin; Adrien Ponticorvo; Anthony J. Durkin; Vasan Venugopalan; Bernard Choi; Bruce J. Tromberg
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Paper Abstract

Baseline optical properties are typically assumed in calculating the differential pathlength factor (DPF) of mouse brains, a value used in the modified Beer–Lambert law to characterize an evoked stimulus response. We used spatial frequency domain imaging to measure in vivo baseline optical properties in 20-month-old control (n=8) and triple transgenic APP/PS1/tau (3xTg-AD) (n=5) mouse brains. Average μa for control and 3xTg-AD mice was 0.82±0.05 and 0.65±0.05  mm1, respectively, at 460 nm; and 0.71±0.04 and 0.55±0.04  mm1, respectively, at 530 nm. Average μs for control and 3xTg-AD mice was 1.5±0.1 and 1.7±0.1  mm1, respectively, at 460 nm; and 1.3±0.1 and 1.5±0.1  mm1, respectively, at 530 nm. The calculated DPF for control and 3xTg-AD mice was 0.58±0.04 and 0.64±0.04 OD mm, respectively, at 460 nm; and 0.66±0.03 and 0.73±0.05 OD mm, respectively, at 530 nm. In hindpaw stimulation experiments, the hemodynamic increase in brain tissue concentration of oxyhemoglobin was threefold larger and two times longer in the control mice compared to 3xTg-AD mice. Furthermore, the washout of deoxyhemoglobin from increased brain perfusion was seven times larger in controls compared to 3xTg-AD mice (p<0.05).

Paper Details

Date Published: 12 October 2015
PDF: 9 pages
2(4) 045001 doi: 10.1117/1.NPh.2.4.045001
Published in: Neurophotonics Volume 2, Issue 4
Show Author Affiliations
Alexander J. Lin, Beckman Laser Institute and Medical Clinic (United States)
Univ. of California, Irvine (United States)
Adrien Ponticorvo, Beckman Laser Institute and Medical Clinic (United States)
Anthony J. Durkin, Beckman Laser Institute and Medical Clinic (United States)
Vasan Venugopalan, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)
Bernard Choi, Beckman Laser Institute and Medical Clinic (United States)
Univ. of California, Irvine (United States)
Bruce J. Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Univ. of California, Irvine (United States)


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