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Neurophotonics • Open Access

Ultramicroscopy: development and outlook
Author(s): Hans-Ulrich Dodt; Saiedeh Saghafi; Klaus Becker; Nina Jährling; Axel Niendorf; Christian Hahn; Marko Pende; Martina Wanis

Paper Abstract

We present an overview of the ultramicroscopy technique we developed. Starting from developments 100 years ago, we designed a light sheet microscope and a chemical clearing to image complete mouse brains. Fluorescence of green fluorescent protein (GFP)-labeled neurons in mouse brains could be preserved with our 3DISCO clearing and high-resolution three-dimensional (3-D) recordings were obtained. Ultramicroscopy was also used to image whole mouse embryos and flies. We improved the optical sectioning of our light sheet microscope by generating longer and thinner light sheets with aspheric optics. To obtain high-resolution images, we corrected available air microscope objectives for clearing solutions with high refractive index. We discuss how eventually super resolution could be realized in light sheet microscopy by applying stimulated emission depletion technology. Also the imaging of brain function by recording of mouse brains expressing cfos-GFP is discussed. Finally, we show the first 3-D recordings of human breast cancer with light sheet microscopy as application in medical diagnostics.

Paper Details

Date Published: 9 November 2015
PDF: 8 pages
2(4) 041407 doi: 10.1117/1.NPh.2.4.041407
Published in: Neurophotonics Volume 2, Issue 4
Show Author Affiliations
Hans-Ulrich Dodt, Technische Univ. Wien (Austria)
Medizinische Univ. Wien (Austria)
Saiedeh Saghafi, Technische Univ. Wien (Austria)
Medizinische Univ. Wien (Austria)
Klaus Becker, Technische Univ. Wien (Austria)
Medizinische Univ. Wien (Austria)
Nina Jährling, Technische Univ. Wien (Austria)
Medizinische Univ. Wien (Austria)
Axel Niendorf, Pathologie Hamburg-West (Germany)
Christian Hahn, Technische Univ. Wien (Austria)
Medizinische Univ. Wien (Austria)
Marko Pende, Technische Univ. Wien (Austria)
Medizinische Univ. Wien (Austria)
Martina Wanis, Technische Univ. Wien (Austria)
Medizinische Univ. Wien (Austria)


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