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Neurophotonics

Fluorescence micro-optical sectioning tomography using acousto-optical deflector-based confocal scheme
Author(s): Xiaoli Qi; Tao Yang; Longhui Li; Jiancun Wang; Shaoqun Zeng; Xiaohua Lv
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Paper Abstract

Fluorescent labeling has opened up the possibility of clarifying the complex distribution and circuit wiring of specific neural circuits for particular functions. To acquire the brain-wide fluorescently labeled neural wiring, we have previously developed the fluorescence micro-optical sectioning tomography imaging system. This employs simultaneous mechanical sectioning and confocal imaging of the slices, and is capable of acquiring the image dataset of a centimeter-sized whole-mouse brain at a voxel resolution of 1  μm. We analyze the key optical considerations for the use of an acousto-optical deflector (AOD) scanner-based confocal detection scheme in this system. As a result, the influence of confocal detection, the imaging site during sectioning, and AOD fast scan mode on signal-to-background noise ratio are described. It is shown that mechanical sectioning to separate the slice and optical sectioning by confocal detection should be combined to maximize background suppression in simultaneous fast scan imaging while sectioning system setup.

Paper Details

Date Published: 15 October 2015
PDF: 7 pages
2(4) 041406 doi: 10.1117/1.NPh.2.4.041406
Published in: Neurophotonics Volume 2, Issue 4
Show Author Affiliations
Xiaoli Qi, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Tao Yang, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Longhui Li, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Jiancun Wang, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)
Xiaohua Lv, Britton Chance Ctr. for Biomedical Photonics (China)
Huazhong Univ. of Science and Technology (China)


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