Share Email Print

Neurophotonics • Open Access

Serotype-dependent transduction efficiencies of recombinant adeno-associated viral vectors in monkey neocortex
Author(s): Annelies Gerits; Pascaline Vancraeyenest; Samme Vreysen; Marie-Eve Laramée; Annelies Michiels; Rik Gijsbers; Chris Van den Haute; Lieve Moons; Zeger Debyser; Veerle Baekelandt; Lutgarde Arckens; Wim Vanduffel

Paper Abstract

Viral vector-mediated expression of genes (e.g., coding for opsins and designer receptors) has grown increasingly popular. Cell-type specific expression is achieved by altering viral vector tropism through crosspackaging or by cell-specific promoters driving gene expression. Detailed information about transduction properties of most recombinant adeno-associated viral vector (rAAV) serotypes in macaque cortex is gradually becoming available. Here, we compare transduction efficiencies and expression patterns of reporter genes in two macaque neocortical areas employing different rAAV serotypes and promoters. A short version of the calmodulin-kinase-II (CaMKIIα0.4) promoter resulted in reporter gene expression in cortical neurons for all tested rAAVs, albeit with different efficiencies for spread: rAAV2/5>>rAAV2/7>rAAV2/8>rAAV2/9>>rAAV2/1 and proportion of transduced cells: rAAV2/1>rAAV2/5>rAAV2/7=rAAV2/9>rAAV2/8. In contrast to rodent studies, the cytomegalovirus (CMV) promoter appeared least efficient in macaque cortex. The human synapsin-1 promoter preceded by the CMV enhancer (enhSyn1) produced homogeneous reporter gene expression across all layers, while two variants of the CaMKIIα promoter resulted in different laminar transduction patterns and cell specificities. Finally, differences in expression patterns were observed when the same viral vector was injected in two neocortical areas. Our results corroborate previous findings that reporter-gene expression patterns and efficiency of rAAV transduction depend on serotype, promoter, cortical layer, and area.

Paper Details

Date Published: 1 October 2015
PDF: 17 pages
Neurophoton. 2(3) 031209 doi: 10.1117/1.NPh.2.3.031209
Published in: Neurophotonics Volume 2, Issue 3
Show Author Affiliations
Annelies Gerits, KU Leuven (Belgium)
Pascaline Vancraeyenest, KU Leuven (Belgium)
Samme Vreysen, KU Leuven (Belgium)
Marie-Eve Laramée, KU Leuven (Belgium)
Annelies Michiels, KU Leuven (Belgium)
Rik Gijsbers, KU Leuven (Belgium)
Chris Van den Haute, KU Leuven (Belgium)
Lieve Moons, KU Leuven (Belgium)
Zeger Debyser, KU Leuven (Belgium)
Veerle Baekelandt, KU Leuven (Belgium)
Lutgarde Arckens, KU Leuven (Belgium)
Wim Vanduffel, KU Leuven (Belgium)
Massachusetts General Hospital (United States)
Harvard Medical School (United States)

© SPIE. Terms of Use
Back to Top