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All-optical bidirectional neural interfacing using hybrid multiphoton holographic optogenetic stimulation
Author(s): Shir Paluch-Siegler; Hod Dana; Tom Mayblum; Inbar Brosh; Inna Gefen; Shy Shoham
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Paper Abstract

Our understanding of neural information processing could potentially be advanced by combining flexible three-dimensional (3-D) neuroimaging and stimulation. Recent developments in optogenetics suggest that neurophotonic approaches are in principle highly suited for noncontact stimulation of network activity patterns. In particular, two-photon holographic optical neural stimulation (2P-HONS) has emerged as a leading approach for multisite 3-D excitation, and combining it with temporal focusing (TF) further enables axially confined yet spatially extended light patterns. Here, we study key steps toward bidirectional cell-targeted 3-D interfacing by introducing and testing a hybrid new 2P-TF-HONS stimulation path for accurate parallel optogenetic excitation into a recently developed hybrid multiphoton 3-D imaging system. The system is shown to allow targeted all-optical probing of <italic<in vitro</italic< cortical networks expressing channelrhodopsin-2 using a regeneratively amplified femtosecond laser source tuned to 905 nm. These developments further advance a prospective new tool for studying and achieving distributed control over 3-D neuronal circuits both <italic<in vitro</italic< and <italic<in vivo</italic<.

Paper Details

Date Published: 23 July 2015
PDF: 9 pages
2(3) 031208 doi: 10.1117/1.NPh.2.3.031208
Published in: Neurophotonics Volume 2, Issue 3
Show Author Affiliations
Shir Paluch-Siegler, Technion-Israel Institute of Technology (Israel)
Hod Dana, Howard Hughes Medical Institute (United States)
Tom Mayblum, Technion-Israel Institute of Technology (Israel)
Inbar Brosh, Technion-Israel Institute of Technology (Israel)
Inna Gefen, Ruppin Academic Ctr. (Israel)
Shy Shoham, Technion-Israel Institute of Technology (Israel)

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