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Modulating dopamine release by optogenetics in transgenic mice reveals terminal dopaminergic dynamics
Author(s): Yao Lu; Nicolette Driscoll; Ilker Ozden; Zeyang Yu; Arto V. Nurmikko
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Paper Abstract

Dopamine (DA) release and uptake dynamics in the nucleus accumbens (NAc) have important implications for neurological diseases and mammalian animal behaviors. We demonstrate here the use of cell-type-specific optogenetic targeting in conjunction with fast-scan cyclic voltammetry applied to brain slices prepared from specifically tailored transgenic mice, which conditionally express channelrhodopsin-2 (ChR2) through dopamine transporter (DAT)-Cre. Terminal dopaminergic dynamics and the direct manipulation of induced DA release level by controlling light intensity, pulse width, and the shape of stimulation waveforms were studied. Effective cell terminal-targeting optogenetic induction of DA release at physiological levels in NAc is demonstrated and discussed. It was found that delivering more light energy by increasing stimulation intensity and length is not the only way to control DA release; the temporal shape of the stimulus waveform at light onset is also critically related to induced DA concentrations. In addition, DA uptake dynamics as well as the recovery of the presynaptic releasable DA pool are studied and modeled. More broadly, our experimental findings provide important further evidence for effectively applying optogenetics to induce neurotransmitter release in the behaviorally relevant region of the brain in a highly cell-type selective context.

Paper Details

Date Published: 9 July 2015
PDF: 10 pages
Neurophoton. 2(3) 031207 doi: 10.1117/1.NPh.2.3.031207
Published in: Neurophotonics Volume 2, Issue 3
Show Author Affiliations
Yao Lu, Brown Univ. (United States)
Nicolette Driscoll, Brown Univ. (United States)
Ilker Ozden, Brown Univ. (United States)
Zeyang Yu, Brown Univ. (United States)
Arto V. Nurmikko, Brown Univ. (United States)

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