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Neurophotonics • Open Access

All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation
Author(s): Shun Qiang Lo; Dawn X. P. Koh; Judy C. G. Sng; George J. Augustine

Paper Abstract

We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate specific layers and columns, while a red-shifted voltage-sensitive dye was used to map out large-scale circuit activity. We demonstrate that such all-optical mapping can interrogate various circuits in somatosensory cortex by sequentially activating different layers and columns. Further, mapping in slices from whisker-deprived mice demonstrated that chronic sensory deprivation did not significantly alter feedforward inhibition driven by layer 5 pyramidal neurons. Further development of voltage-sensitive optical probes should allow this all-optical mapping approach to become an important and high-throughput tool for mapping circuit interactions in the brain.

Paper Details

Date Published: 31 March 2015
PDF: 11 pages
2(2) 021013 doi: 10.1117/1.NPh.2.2.021013
Published in: Neurophotonics Volume 2, Issue 2
Show Author Affiliations
Shun Qiang Lo, National Univ. of Singapore (Singapore)
Nanyang Technological Univ. (Singapore)
Institute of Molecular and Cell Biology (Singapore)
Dawn X. P. Koh, National Univ. of Singapore (Singapore)
National Univ. of Singapore (Singapore)
Singapore Institute of Clinical Sciences (SICS) (Singapore)
Judy C. G. Sng, National Univ. of Singapore (Singapore)
Singapore Institute of Clinical Sciences (SICS) (Singapore)
George J. Augustine, The Lee Kong Chian School of Medicine (Singapore)
Nanyang Technological Univ. (Singapore)
Institute of Molecular and Cell Biology (Singapore)


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