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Neurophotonics • Open Access

Route to genetically targeted optical electrophysiology: development and applications of voltage-sensitive fluorescent proteins
Author(s): Walther Akemann; Chenchen Song; Hiroki Mutoh; Thomas Knöpfel

Paper Abstract

The invention of membrane voltage protein indicators widens the reach of optical voltage imaging in cell physiology, most notably neurophysiology, by enabling membrane voltage recordings from genetically defined cell types in chronic and life-long preparations. While the last years have seen a dramatic improvement in the technical performance of these indicators, concomitant innovations in optogenetics, optical axon tracing, and high-speed digital microscopy are beginning to fulfill the age-old vision of an all-optical analysis of neuronal circuits, reaching beyond the limits of traditional electrode-based recordings. We will present our personal account of the development of protein voltage indicators from the pioneering days to the present state, including their applications in neurophysiology that has inspired our own work for more than a decade.

Paper Details

Date Published: 14 January 2015
PDF: 9 pages
2(2) 021008 doi: 10.1117/1.NPh.2.2.021008
Published in: Neurophotonics Volume 2, Issue 2
Show Author Affiliations
Walther Akemann, Imperial College London (United Kingdom)
Institute of Biology (France)
Chenchen Song, Imperial College London (United Kingdom)
Hiroki Mutoh, Hamamatsu Univ. School of Medicine (Japan)
Thomas Knöpfel, Imperial College London (United Kingdom)


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