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Neurophotonics

Multimodal reconstruction of microvascular-flow distributions using combined two-photon microscopy and Doppler optical coherence tomography
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Paper Abstract

Computing microvascular cerebral blood flow (μCBF) in real cortical angiograms is challenging. Here, we investigated whether the use of Doppler optical coherence tomography (DOCT) flow measurements in individual vessel segments can help in reconstructing μCBF across the entire vasculature of a truncated cortical angiogram. A μCBF computational framework integrating DOCT measurements is presented. Simulations performed on a synthetic angiogram showed that the addition of DOCT measurements, especially close to large inflowing or outflowing vessels, reduces the impact of pressure boundary conditions and estimated vessel resistances resulting in a more accurate reconstruction of μCBF. Our technique was then applied to reconstruct microvascular flow distributions in the mouse cortex down to 660  μm by combining two-photon laser scanning microscopy angiography with DOCT.

Paper Details

Date Published: 12 March 2015
PDF: 8 pages
2(1) 015008 doi: 10.1117/1.NPh.2.1.015008
Published in: Neurophotonics Volume 2, Issue 1
Show Author Affiliations
Louis Gagnon, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Harvard-MIT Health Sciences and Technology (United States)
Ecole Polytechnique de Montréal (Canada)
Sava Sakadžić, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Fréderic Lesage, Ecole Polytechnique de Montréal (Canada)
Emiri T. Mandeville, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Qianqian Fang, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Mohammad A. Yaseen, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
David A. Boas, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Harvard-MIT Health Sciences and Technology (United States)


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