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Neurophotonics

Optical coherence tomography visualizes neurons in human entorhinal cortex
Author(s): Caroline Magnain; Jean C. Augustinack; Ender Konukoglu; Matthew P. Frosch; Sava Sakadžić; Ani Varjabedian; Nathalie Garcia; Van J. Wedeen; David A. Boas; Bruce Fischl
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Paper Abstract

The cytoarchitecture of the human brain is of great interest in diverse fields: neuroanatomy, neurology, neuroscience, and neuropathology. Traditional histology is a method that has been historically used to assess cell and fiber content in the ex vivo human brain. However, this technique suffers from significant distortions. We used a previously demonstrated optical coherence microscopy technique to image individual neurons in several square millimeters of en-face tissue blocks from layer II of the human entorhinal cortex, over 50  μm in depth. The same slices were then sectioned and stained for Nissl substance. We registered the optical coherence tomography (OCT) images with the corresponding Nissl stained slices using a nonlinear transformation. The neurons were then segmented in both images and we quantified the overlap. We show that OCT images contain information about neurons that is comparable to what can be obtained from Nissl staining, and thus can be used to assess the cytoarchitecture of the ex vivo human brain with minimal distortion. With the future integration of a vibratome into the OCT imaging rig, this technique can be scaled up to obtain undistorted volumetric data of centimeter cube tissue blocks in the near term, and entire human hemispheres in the future.

Paper Details

Date Published: 9 February 2015
PDF: 8 pages
2(1) 015004 doi: 10.1117/1.NPh.2.1.015004
Published in: Neurophotonics Volume 2, Issue 1
Show Author Affiliations
Caroline Magnain, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Jean C. Augustinack, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Ender Konukoglu, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Matthew P. Frosch, Massachusetts General Hospital (United States)
Sava Sakadžić, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Ani Varjabedian, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Nathalie Garcia, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Van J. Wedeen, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
David A. Boas, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Bruce Fischl, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Massachusetts Institute of Technology (United States)


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