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Neurophotonics • Open Access

Wide-field <italic<in vivo</italic< neocortical calcium dye imaging using a convection-enhanced loading technique combined with simultaneous multiwavelength imaging of voltage-sensitive dyes and hemodynamic signals
Author(s): Hongtao Ma; Samuel Harris; Redi Rahmani; Clay O. Lacefield; Mingrui Zhao; Andy G. Daniel; Zhiping Zhou; Randy M. Bruno; Jason Berwick; Theodore H. Schwartz

Paper Abstract

<italic<In vivo</italic< calcium imaging is an incredibly powerful technique that provides simultaneous information on fast neuronal events, such as action potentials and subthreshold synaptic activity, as well as slower events that occur in the glia and surrounding neuropil. Bulk-loading methods that involve multiple injections can be used for single-cell as well as wide-field imaging studies. However, multiple injections result in inhomogeneous loading as well as multiple sites of potential cortical injury. We used convection-enhanced delivery to create smooth, continuous loading of a large area of the cortical surface through a solitary injection site and demonstrated the efficacy of the technique using confocal microscopy imaging of single cells and physiological responses to single-trial events of spontaneous activity, somatosensory-evoked potentials, and epileptiform events. Combinations of calcium imaging with voltage-sensitive dye and intrinsic signal imaging demonstrate the utility of this technique in neurovascular coupling investigations. Convection-enhanced loading of calcium dyes may be a useful technique to advance the study of cortical processing when widespread loading of a wide-field imaging is required.

Paper Details

Date Published: 24 July 2014
PDF: 12 pages
1(1) 015003 doi: 10.1117/1.NPh.1.1.015003
Published in: Neurophotonics Volume 1, Issue 1
Show Author Affiliations
Hongtao Ma, Weill Cornell Medical College (United States)
Samuel Harris, Weill Cornell Medical College (United States)
Univ. of Sheffield (United Kingdom)
Redi Rahmani, Weill Cornell Medical College (United States)
Geisel School of Medicine at Dartmouth (United States)
Clay O. Lacefield, Columbia Univ. (United States)
Mingrui Zhao, Weill Cornell Medical College (United States)
Andy G. Daniel, Weill Cornell Medical College (United States)
Zhiping Zhou, Weill Cornell Medical College (United States)
Randy M. Bruno, Columbia Univ. (United States)
Jason Berwick, The Univ. of Sheffield (United Kingdom)
Theodore H. Schwartz, Weill Cornell Medical College (United States)

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