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Characterization of the BAC Id3-enhanced green fluorescent protein transgenic mouse line for <italic<in vivo</italic< imaging of astrocytes
Author(s): Cassandra Lamantia; Marie-Eve Tremblay; Ania Majewska
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Paper Abstract

Astrocytes are highly ramified glial cells with critical roles in brain physiology and pathology. Recently, breakthroughs in imaging technology have expanded our understanding of astrocyte function <italic<in vivo</italic<. The <italic<in vivo</italic< study of astrocytic dynamics, however, is limited by the tools available to label astrocytes and their processes. Here, we characterize the bacterial artificial chromosome transgenic Id3-EGFP knock-in mouse to establish its usefulness for <italic<in vivo</italic< imaging of astrocyte processes. Using fixed brain sections, we observed enhanced green fluorescent protein expression in astrocytes and blood vessel walls throughout the brain, although the extent and cell type specificity of expression depended on the brain area and developmental age. Using <italic<in vivo</italic< two-photon imaging, we visualized astrocytes in cortical layers 1–3 in both thin skull and window preparations. In adult animals, astrocytic cell bodies and fine processes could be followed over many hours. Our results suggest that Id3 mice could be used for <italic<in vivo</italic< imaging of astrocytes and blood vessels in development and adulthood.

Paper Details

Date Published: 12 September 2014
PDF: 10 pages
1(1) 011014 doi: 10.1117/1.NPh.1.1.011014
Published in: Neurophotonics Volume 1, Issue 1
Show Author Affiliations
Cassandra Lamantia, Univ. of Rochester (United States)
Marie-Eve Tremblay, Univ. of Rochester (United States)
Ania Majewska, Univ. of Rochester (United States)

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