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Neurophotonics • Open Access

Single-cell imaging tools for brain energy metabolism: a review
Author(s): Alejandro San Martín; Tamara Sotelo-Hitschfeld; Rodrigo Lerchundi; Ignacio Fernández-Moncada; Sebastian Ceballo; Rocío Valdebenito; Felipe Baeza-Lehnert; Karin Alegría; Yasna Contreras-Baeza; Pamela Garrido-Gerter; Ignacio Romero-Gómez; L. Felipe Barros

Paper Abstract

Neurophotonics comes to light at a time in which advances in microscopy and improved calcium reporters are paving the way toward high-resolution functional mapping of the brain. This review relates to a parallel revolution in metabolism. We argue that metabolism needs to be approached both <italic<in vitro</italic< and <italic<in vivo</italic<, and that it does not just exist as a low-level platform but is also a relevant player in information processing. In recent years, genetically encoded fluorescent nanosensors have been introduced to measure glucose, glutamate, ATP, NADH, lactate, and pyruvate in mammalian cells. Reporting relative metabolite levels, absolute concentrations, and metabolic fluxes, these sensors are instrumental for the discovery of new molecular mechanisms. Sensors continue to be developed, which together with a continued improvement in protein expression strategies and new imaging technologies, herald an exciting era of high-resolution characterization of metabolism in the brain and other organs.

Paper Details

Date Published: 29 May 2014
PDF: 9 pages
1(1) 011004 doi: 10.1117/1.NPh.1.1.011004
Published in: Neurophotonics Volume 1, Issue 1
Show Author Affiliations
Alejandro San Martín, Ctr. de Estudios Cientificos (Chile)
Univ. Austral de Chile (Chile)
Tamara Sotelo-Hitschfeld, Ctr. de Estudios Cientificos (Chile)
Univ. Austral de Chile (Chile)
Rodrigo Lerchundi, Ctr. de Estudios Cientificos (Chile)
Univ. Austral de Chile (Chile)
Ignacio Fernández-Moncada, Ctr. de Estudios Cientificos (Chile)
Univ. Austral de Chile (Chile)
Sebastian Ceballo, Ctr. de Estudios Cientificos (Chile)
Rocío Valdebenito, Ctr. de Estudios Cientificos (Chile)
Felipe Baeza-Lehnert, Ctr. de Estudios Cientificos (Chile)
Karin Alegría, Ctr. de Estudios Cientificos (Chile)
Yasna Contreras-Baeza, Ctr. de Estudios Cientificos (Chile)
Univ. Austral de Chile (Chile)
Pamela Garrido-Gerter, Ctr. de Estudios Cientificos (Chile)
Univ. Austral de Chile (Chile)
Ignacio Romero-Gómez, Ctr. de Estudios Cientificos (Chile)
Univ. Austral de Chile (Chile)
L. Felipe Barros, Ctr. de Estudios Cientificos (Chile)


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