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Neurophotonics • Open Access

Programmable wireless light-emitting diode stimulator for chronic stimulation of optogenetic molecules in freely moving mice
Author(s): Mitsuhiro Hashimoto; Akihiro Hata; Takaki Miyata; Hajime Hirase

Paper Abstract

We produced a miniaturized, multicode, multiband, and programmable light-emitting diode (LED) stimulator for wireless control of optogenetic experiments. The LED stimulator is capable of driving three independent LEDs upon reception of an infrared (IR) signal generated by a custom-made IR transmitter. Individual LED photopulse patterns are assigned to different codes of the IR signals (up to 256 codes). The photopulse patterns can be programmed in the on-board microcontroller by specifying the parameters of duration (<1  ms ), frequency (<500  Hz ), and pulse width (<1  ms ). The IR signals were modulated at multiple carrier frequencies to establish multiband IR transmission. Using these devices, we could remotely control the moving direction of a Thy1-ChR2-YFP transgenic mouse by transcranially illuminating the corresponding hemisphere of the primary motor cortex. IR transmitter and LED stimulator will be particularly useful in experiments where free movement or patterned concurrent stimulation is desired, such as testing social communication of rodents.

Paper Details

Date Published: 28 May 2014
PDF: 11 pages
1(1) 011002 doi: 10.1117/1.NPh.1.1.011002
Published in: Neurophotonics Volume 1, Issue 1
Show Author Affiliations
Mitsuhiro Hashimoto, Nagoya Univ. Graduate School of Medicine (Japan)
Akihiro Hata, Fitdesign LLC (Japan)
Takaki Miyata, Nagoya Univ. Graduate School of Medicine (Japan)
Hajime Hirase, RIKEN Brain Science Institute (Japan)


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