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Journal of Nanophotonics • Open Access

Aggregation of nanoparticles in endosomes and lysosomes produces surface-enhanced Raman spectroscopy
Author(s): Leanne Lucas; Xiaoke K. Chen; Aaron J. Smith; Mladen Korbelik; Haishan Zeng; Patrick W. K. Lee; Kevin C. Hewitt

Paper Abstract

The purpose of this study was to explore the use of surface-enhanced Raman spectroscopy (SERS) to image the distribution of epidermal growth factor receptor (EGFR) in cells. To accomplish this task, 30-nm gold nanoparticles (AuNPs) tagged with antibodies to EGFR (1012  per mL) were incubated with cells (106  per mL) of the A431 human epidermoid carcinoma and normal human bronchial epithelial cell lines. Using the 632.8-nm excitation line of a He-Ne laser, Raman spectroscopy measurements were performed using a point mapping scheme. Normal cells show little to no enhancement. SERS signals were observed inside the cytoplasm of A431 cells with an overall enhancement of 4 to 7 orders of magnitude. Raman intensity maps of the 1450 and 1583  cm1 peaks correlate well with the expected distribution of EGFR and AuNPs, aggregated following uptake by endosomes and lysosomes. Spectral features from tyrosine and tryptophan residues dominate the SERS signals.

Paper Details

Date Published: 23 January 2015
PDF: 14 pages
J. Nanophoton. 9(1) 093094 doi: 10.1117/1.JNP.9.093094
Published in: Journal of Nanophotonics Volume 9, Issue 1
Show Author Affiliations
Leanne Lucas, Dalhousie Univ. (Canada)
Xiaoke K. Chen, Simon Fraser Univ. (Canada)
Aaron J. Smith, Dalhousie Univ. (Canada)
Mladen Korbelik, The BC Cancer Agency Research Ctr. (Canada)
Haishan Zeng, The BC Cancer Agency Research Ctr. (Canada)
Patrick W. K. Lee, Dalhousie Univ. (Canada)
Kevin C. Hewitt, Dalhousie Univ. (Canada)

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