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Journal of Micro/Nanolithography, MEMS, and MOEMS

One-step fabrication of three-dimensional polydimethylsiloxane peel-off microwell array for cell trapping
Author(s): Ning Xue; Leonidas Bleris; Jeong-Bong Lee
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Paper Abstract

A three-dimensional polydimethylsiloxane (PDMS)-based microarray was one-step fabricated by releasing from an SU-8 mold for cell trapping. A human embryonic kidney-293 cell line was cultured on the PDMS microarray. By taking advantage of the chemical nature difference between PDMS surface released from oxidized silicon and released from SU-8 substrate, cells can only grow and be attached inside the PDMS microarray after 2 days of cell culture. To investigate the substantial nature of the cell adhesion and repellency on the PDMS substrate, atomic force microscopy, contact angle, and x-ray photoelectron spectroscopy methods have been carried out to analyze the physical and chemical properties of the PDMS material surfaces under different surface treatment circumstances.

Paper Details

Date Published: 19 February 2015
PDF: 7 pages
J. Micro/Nanolith. 14(1) 014503 doi: 10.1117/1.JMM.14.1.014503
Published in: Journal of Micro/Nanolithography, MEMS, and MOEMS Volume 14, Issue 1
Show Author Affiliations
Ning Xue, The Univ. of Texas at Dallas (United States)
Institute of Microelectronics (Singapore)
Leonidas Bleris, The Univ. of Texas at Dallas (United States)
Jeong-Bong Lee, The Univ. of Texas at Dallas (United States)


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