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Journal of Micro/Nanolithography, MEMS, and MOEMS

Total internal reflection–based module for fluorescence and absorbance detection
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Paper Abstract

We present a miniaturized polymer-based micro-optical detection unit for ultraviolet and visible laser-induced fluorescence (LIF) and absorbance (ABS) analysis with an interaction length of 3 mm. We use nonsequential optical ray tracing simulations to model the system and to optimize its performance with respect to optical efficiency and system complexity. The design features a compact optical system combining total internal reflection (TIR) mirrors and refractive optics. The detection module is prototyped with deep proton writing in 2-mm-thick polymethylmethacrylate and quantitatively characterized using a three-dimensional coordinate measurement machine. We demonstrate the proof-of-concept of this TIR mirror–based module for coumarin 480 obtaining limits of detection of 50 pM and 500 nM for LIF and ABS, respectively.

Paper Details

Date Published: 3 July 2014
PDF: 9 pages
J. Micro/Nanolith. MEMS MOEMS 13(3) 033001 doi: 10.1117/1.JMM.13.3.033001
Published in: Journal of Micro/Nanolithography, MEMS, and MOEMS Volume 13, Issue 3
Show Author Affiliations
Tom Verschooten, Vrije Univ. Brussel (Belgium)
Heidi Ottevaere, Vrije Univ. Brussel (Belgium)
Michael Vervaeke, Vrije Univ. Brussel (Belgium)
Jürgen Van Erps, Vrije Univ. Brussel (Belgium)
Hugo Thienpont, Vrije Univ. Brussel (Belgium)

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