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Journal of Micro/Nanolithography, MEMS, and MOEMS

Development of microarray device for functional evaluation of PC12D cell axonal extension ability
Author(s): Eiji Nakamachi; Junpei Yanagimoto; Shinya Murakami; Yusuke Morita
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Paper Abstract

In this study, we developed a microarray bio-MEMS device that could trap PC12D (rat pheochromocytoma cells) cells to examine the intercellular interaction effect on the cell activation and the axonal extension ability. This is needed to assign particular patterns of PC12D cells to establish a cell functional evaluation technique. This experimental observation-based technique can be used for design of the cell sheet and scaffold for peripheral and central nerve regeneration. We have fabricated a micropillar-array bio-MEMS device, whose diameter was approximately 10 μm, by using thick photoresist SU-8 on the glass slide substrate. A maximum trapped PC12D cell ratio, 48.5%, was achieved. Through experimental observation of patterned PC12D “bi-cells” activation, we obtained the following results. Most of the PC12D “bi-cells” which had distances between 40 and 100 μm were connected after 24 h with a high probability. On the other hand, “bi-cells” which had distances between 110 and 200 μm were not connected. In addition, we measured axonal extension velocities in cases where the intercellular distance was between 40 and 100 μm. A maximum axonal extension velocity, 86.4  μm/h, was obtained at the intercellular distance of 40 μm.

Paper Details

Date Published: 27 June 2014
PDF: 9 pages
J. Micro/Nanolith. 13(2) 023013 doi: 10.1117/1.JMM.13.2.023013
Published in: Journal of Micro/Nanolithography, MEMS, and MOEMS Volume 13, Issue 2
Show Author Affiliations
Eiji Nakamachi, Doshisha Univ. (Japan)
Junpei Yanagimoto, Doshisha Univ. (Japan)
Shinya Murakami, Doshisha Univ. (Japan)
Yusuke Morita, Doshisha Univ. (Japan)


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