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Journal of Micro/Nanolithography, MEMS, and MOEMS

Development path and current status of the NANIVID: a new device for cancer cell studies
Author(s): Waseem Khan Raja; Michael R. Padgen; James K. Williams; James Castracane; Frank B. Gertler; Jeffrey Wyckoff; John S. Condeelis
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Paper Abstract

Cancer cells create a unique microenvironment in vivo that enables migration to distant organs. To better understand the tumor microenvironment, special tools and devices are required to monitor the interactions between different cell types and the effects of particular chemical gradients. Our study presents the design and optimization of a versatile chemotaxis device, the nano-intravital device (NANIVID), which consists of etched and bonded glass substrates that create a soluble factor reservoir. The device contains a customized hydrogel blend that is loaded with epidermal growth factor (EGF), which diffuses from the outlet to create a chemotactic gradient that can be sustained for many hours in order to attract specific cells to the device. A microelectrode array is under development for quantification of cell collection and will be incorporated into future device generations. Additionally, the NANIVID can be modified to generate gradients of other soluble factors in order to initiate controlled changes to the microenvironment including the induction of hypoxia, manipulation of extracellular matrix stiffness, etc. The focus of the article is to present the design and optimization of the device towards wide ranging applications of cancer cell dynamics in vitro and, ultimately, implantation for in vivo investigations.

Paper Details

Date Published: 29 March 2012
PDF: 10 pages
J. Micro/Nanolith. 11(1) 013013 doi: 10.1117/1.JMM.11.1.013013
Published in: Journal of Micro/Nanolithography, MEMS, and MOEMS Volume 11, Issue 1
Show Author Affiliations
Waseem Khan Raja, Tufts Univ. (United States)
Michael R. Padgen, Univ. at Albany (United States)
James K. Williams, Univ. at Albany (United States)
James Castracane, Univ. at Albany (United States)
Frank B. Gertler, Massachusetts Institute of Technology (United States)
Jeffrey Wyckoff, Albert Einstein College of Medicine of Yeshiva Univ. (United States)
John S. Condeelis, Albert Einstein College of Medicine of Yeshiva Univ. (United States)

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