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Journal of Medical Imaging • Open Access

Quantitative analysis of ex vivo colorectal epithelium using an automated feature extraction algorithm for microendoscopy image data
Author(s): Sandra P. Prieto; Keith K. Lai; Jonathan A. Laryea; Jason S. Mizell; Timothy J. Muldoon

Paper Abstract

Qualitative screening for colorectal polyps via fiber bundle microendoscopy imaging has shown promising results, with studies reporting high rates of sensitivity and specificity, as well as low interobserver variability with trained clinicians. A quantitative image quality control and image feature extraction algorithm (QFEA) was designed to lessen the burden of training and provide objective data for improved clinical efficacy of this method. After a quantitative image quality control step, QFEA extracts field-of-view area, crypt area, crypt circularity, and crypt number per image. To develop and validate this QFEA, a training set of microendoscopy images was collected from freshly resected porcine colon epithelium. The algorithm was then further validated on ex vivo image data collected from eight human subjects, selected from clinically normal appearing regions distant from grossly visible tumor in surgically resected colorectal tissue. QFEA has proven flexible in application to both mosaics and individual images, and its automated crypt detection sensitivity ranges from 71 to 94% despite intensity and contrast variation within the field of view. It also demonstrates the ability to detect and quantify differences in grossly normal regions among different subjects, suggesting the potential efficacy of this approach in detecting occult regions of dysplasia.

Paper Details

Date Published: 3 June 2016
PDF: 13 pages
J. Med. Img. 3(2) 024502 doi: 10.1117/1.JMI.3.2.024502
Published in: Journal of Medical Imaging Volume 3, Issue 2
Show Author Affiliations
Sandra P. Prieto, Univ. of Arkansas (United States)
Keith K. Lai, Univ. of Arkansas for Medical Sciences (United States)
Jonathan A. Laryea, Univ. of Arkansas for Medical Sciences (United States)
Jason S. Mizell, Univ. of Arkansas for Medical Sciences (United States)
Timothy J. Muldoon, Univ. of Arkansas (United States)


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