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Journal of Medical Imaging

Multipurpose nonlinear optical imaging system for <italic<in vivo</italic< and <italic<ex vivo</italic< multimodal histology
Author(s): Martin Weinigel; Hans G. Breunig; Aisada Uchugonova; Karsten König
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Paper Abstract

We report on a flexible multipurpose nonlinear microscopic imaging system based on a femtosecond excitation source and a photonic crystal fiber with multiple miniaturized time-correlated single-photon counting detectors. The system provides the simultaneous acquisition of e.g., two-photon autofluorescence, second-harmonic generation, and coherent anti-Stokes Raman scattering images. Its flexible scan head permits <italic<ex vivo</italic< biological imaging with subcellular resolution such as rapid biopsy examination during surgery as well as imaging on small as well as large animals. Above all, such an arrangement perfectly matches the needs for the clinical investigation of human skin <italic<in vivo</italic< where knowledge about the distribution of endogenous fluorophores, second-harmonic generation–active collagen as well as nonfluorescent lipids is of high interest.

Paper Details

Date Published: 4 March 2015
PDF: 10 pages
J. Med. Img. 2(1) 016003 doi: 10.1117/1.JMI.2.1.016003
Published in: Journal of Medical Imaging Volume 2, Issue 1
Show Author Affiliations
Martin Weinigel, JenLab GmbH (Germany)
Hans G. Breunig, JenLab GmbH (Germany)
Univ. des Saarlandes (Germany)
Aisada Uchugonova, JenLab GmbH (Germany)
Univ. des Saarlandes (Germany)
Karsten König, JenLab GmbH (Germany)
Univ. des Saarlandes (Germany)

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