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Journal of Biomedical Optics • Open Access

Benchtop and animal validation of a portable fluorescence microscopic imaging system for potential use in cholecystectomy

Paper Abstract

We propose a portable fluorescence microscopic imaging system (PFMS) for intraoperative display of biliary structure and prevention of iatrogenic injuries during cholecystectomy. The system consists of a light source module, a camera module, and a Raspberry Pi computer with an LCD. Indocyanine green (ICG) is used as a fluorescent contrast agent for experimental validation of the system. Fluorescence intensities of the ICG aqueous solution at different concentration levels are acquired by our PFMS and compared with those of a commercial Xenogen IVIS system. We study the fluorescence detection depth by superposing different thicknesses of chicken breast on an ICG-loaded agar phantom. We verify the technical feasibility for identifying potential iatrogenic injury in cholecystectomy using a rat model in vivo. The proposed PFMS system is portable, inexpensive, and suitable for deployment in resource-limited settings.

Paper Details

Date Published: 22 February 2018
PDF: 4 pages
J. Biomed. Opt. 23(2) 020504 doi: 10.1117/1.JBO.23.2.020504
Published in: Journal of Biomedical Optics Volume 23, Issue 2
Show Author Affiliations
Jian Ye, Univ. of Science and Technology of China (China)
Guanghui Liu, Univ. of Science and Technology of China (China)
Peng Liu, Univ. of Science and Technology of China (China)
Shiwu Zhang, Univ. of Science and Technology of China (China)
Pengfei Shao, Univ. of Science and Technology of China (China)
Zachary J. Smith, Univ. of Science and Technology of China (China)
Chenhai Liu, Anhui Provincial Hospital (China)
Ronald X. Xu, Univ. of Science and Technology of China (China)
The Ohio State Univ. (United States)


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