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Journal of Biomedical Optics • Open Access

Fiber bundle endomicroscopy with multi-illumination for three-dimensional reflectance image reconstruction
Author(s): Yoriko Ando; Hirohito Sawahata; Takeshi Kawano; Kowa Koida; Rika Numano

Paper Abstract

Bundled fiber optics allow in vivo imaging at deep sites in a body. The intrinsic optical contrast detects detailed structures in blood vessels and organs. We developed a bundled-fiber-coupled endomicroscope, enabling stereoscopic three-dimensional (3-D) reflectance imaging with a multipositional illumination scheme. Two illumination sites were attached to obtain reflectance images with left and right illumination. Depth was estimated by the horizontal disparity between the two images under alternative illuminations and was calibrated by the targets with known depths. This depth reconstruction was applied to an animal model to obtain the 3-D structure of blood vessels of the cerebral cortex (Cereb cortex) and preputial gland (Pre gla). The 3-D endomicroscope could be instrumental to microlevel reflectance imaging, improving the precision in subjective depth perception, spatial orientation, and identification of anatomical structures.

Paper Details

Date Published: 16 February 2018
PDF: 4 pages
J. Biomed. Opt. 23(2) 020502 doi: 10.1117/1.JBO.23.2.020502
Published in: Journal of Biomedical Optics Volume 23, Issue 2
Show Author Affiliations
Yoriko Ando, Toyohashi Univ. of Technology (Japan)
Hirohito Sawahata, Toyohashi Univ. of Technology (Japan)
Takeshi Kawano, Toyohashi Univ. of Technology (Japan)
Kowa Koida, Toyohashi Univ. of Technology (Japan)
Rika Numano, Toyohashi Univ. of Technology (Japan)


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