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Journal of Biomedical Optics • new

Intrinsic fluorescence for cervical precancer detection using polarized light based in-house fabricated portable device
Author(s): Bharat Lal Meena; Pankaj Singh; Amar Nath Sah; Kiran Pandey; Asha Agarwal; Chayanika Pantola; Asima Pradhan
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Paper Abstract

An in-house fabricated portable device has been tested to detect cervical precancer through the intrinsic fluorescence from human cervix of the whole uterus in a clinical setting. A previously validated technique based on simultaneously acquired polarized fluorescence and polarized elastic scattering spectra from a turbid medium is used to extract the intrinsic fluorescence. Using a diode laser at 405 nm, intrinsic fluorescence of flavin adenine dinucleotide, which is the dominant fluorophore and other contributing fluorophores in the epithelium of cervical tissue, has been extracted. Different grades of cervical precancer (cervical intraepithelial neoplasia; CIN) have been discriminated using principal component analysis-based Mahalanobis distance and linear discriminant analysis. Normal, CIN I and CIN II samples have been discriminated from one another with high sensitivity and specificity at 95% confidence level. This ex vivo study with cervix of whole uterus samples immediately after hysterectomy in a clinical environment indicates that the in-house fabricated portable device has the potential to be used as a screening tool for in vivo precancer detection using intrinsic fluorescence.

Paper Details

Date Published: 16 January 2018
PDF: 7 pages
J. Biomed. Opt. 23(1) 015005 doi: 10.1117/1.JBO.23.1.015005
Published in: Journal of Biomedical Optics Volume 23, Issue 1
Show Author Affiliations
Bharat Lal Meena, Indian Institute of Technology Kanpur (India)
Univ. of Rajasthan, Jaipur (India)
Pankaj Singh, Indian Institute of Technology Kanpur (India)
LSM Government PG College (India)
Amar Nath Sah, Indian Institute of Technology Kanpur (India)
Kiran Pandey, Ganesh Shankar Vidyarthi Memorial Medical College (India)
Asha Agarwal, Regency Hospital (India)
Chayanika Pantola, L.P.S. Institute of Cardiology (India)
Asima Pradhan, Indian Institute of Technology Kanpur (India)


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