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Journal of Biomedical Optics

Determination of the complex refractive index segments of turbid sample with multispectral spatially modulated structured light and models approximation
Author(s): Omri Meitav; Oren Shaul; David Abookasis
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Paper Abstract

Spectral data enabling the derivation of a biological tissue sample’s complex refractive index (CRI) can provide a range of valuable information in the clinical and research contexts. Specifically, changes in the CRI reflect alterations in tissue morphology and chemical composition, enabling its use as an optical marker during diagnosis and treatment. In the present work, we report a method for estimating the real and imaginary parts of the CRI of a biological sample using Kramers–Kronig (KK) relations in the spatial frequency domain. In this method, phase-shifted sinusoidal patterns at single high spatial frequency are serially projected onto the sample surface at different near-infrared wavelengths while a camera mounted normal to the sample surface acquires the reflected diffuse light. In the offline analysis pipeline, recorded images at each wavelength are converted to spatial phase maps using KK analysis and are then calibrated against phase-models derived from diffusion approximation. The amplitude of the reflected light, together with phase data, is then introduced into Fresnel equations to resolve both real and imaginary segments of the CRI at each wavelength. The technique was validated in tissue-mimicking phantoms with known optical parameters and in mouse models of ischemic injury and heat stress. Experimental data obtained indicate variations in the CRI among brain tissue suffering from injury. CRI fluctuations correlated with alterations in the scattering and absorption coefficients of the injured tissue are demonstrated. This technique for deriving dynamic changes in the CRI of tissue may be further developed as a clinical diagnostic tool and for biomedical research applications. To the best of our knowledge, this is the first report of the estimation of the spectral CRI of a mouse head following injury obtained in the spatial frequency domain.

Paper Details

Date Published: 28 September 2017
PDF: 10 pages
J. Biomed. Opt. 22(9) 097004 doi: 10.1117/1.JBO.22.9.097004
Published in: Journal of Biomedical Optics Volume 22, Issue 9
Show Author Affiliations
Omri Meitav, Ariel Univ. (Israel)
Oren Shaul, Ariel Univ. (Israel)
David Abookasis, Ariel Univ. (Israel)

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