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Journal of Biomedical Optics

FRET-based method for evaluation of the efficiency of reversible and irreversible sonoporation
Author(s): Paulius Ruzgys; Mindaugas Tamošiūnas; Vanesa Lukinsone; Saulius Šatkauskas
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Paper Abstract

It is widely known that not all of the treated cells survive after introduction of exogenous molecules via any physical method. Therefore, it is important to develop methods that would allow simultaneous evaluation of both molecular delivery efficiency and cell viability. This study presents Förster resonance energy transfer (FRET)-based method that allows molecular transfer and cell viability evaluation in a single measurement by employing two common fluorescent dyes, namely, ethidium bromide and trypan blue. The method has been validated using cell sonoporation. The FRET-based method allows the efficiency evaluation of both reversible and irreversible sonoporation in a single experiment. Therefore, this method could be used to reduce time, labor, and material cost while improving the accuracy of evaluations.

Paper Details

Date Published: 14 September 2017
PDF: 6 pages
J. Biomed. Opt. 22(9) 097001 doi: 10.1117/1.JBO.22.9.097001
Published in: Journal of Biomedical Optics Volume 22, Issue 9
Show Author Affiliations
Paulius Ruzgys, Vytautas Magnus Univ. (Lithuania)
Mindaugas Tamošiūnas, Vytautas Magnus Univ. (Lithuania)
Vanesa Lukinsone, Univ. of Latvia (Latvia)
Saulius Šatkauskas, Vytautas Magnus Univ. (Lithuania)

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