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Journal of Biomedical Optics

Red fluorescence imaging for dental plaque detection and quantification: pilot study
Author(s): Zhao Liu; Juliana Gomez; Soniya Khan; Deborah Peru; Roger Ellwood
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Paper Abstract

The red fluorescence of dental plaque originating from porphyrins in oral bacteria may allow visualization, detection, and scoring of plaque without disclosing agents. Two studies were conducted. The first included 24 healthy participants who abstained from oral hygiene for 24 h. Dental plaque was collected from tooth surfaces, and a 10% solution was prepared. These were scanned by a molecular spectrometer to identify the optimum excitation and emission wavelengths of plaque for developing a red fluorescence imaging system. Fourteen healthy subjects completed the second study. After a washout period (1 week), participants had a prophylaxis at baseline and abstained from oral hygiene during the study. They were monitored using the fluorescence imaging system at baseline, 24 h, and 48 h. A dentist clinically assessed plaque after disclosing and on red fluorescence images. Three descriptors were extracted from images and a RUSBoost classifier derived computer fluorescence scores through cross-validation. Red fluorescence plaque levels increased during the 48-h accumulation. Plaque progression was identified by dentist assessment and computer analysis, presenting significant differences between visits at tooth and subject levels (p<0.05). Moderate correlations showed between clinical plaque and red fluorescence plaque (r=0.62 dentist, r=0.55 computer). The best agreement was observed when disclosing plaque threshold at level 2, for both dentist evaluation (sensitivity 71.1%, specificity 67.7%, accuracy 70.2%) and computer classification (sensitivity 68.4%, specificity 62.9%, accuracy 67.1%). Given the correlation with clinical diagnosis, red fluorescence imaging shows its potential for providing an objective and promising method for proper oral hygiene assessment.

Paper Details

Date Published: 18 September 2017
PDF: 10 pages
J. Biomed. Opt. 22(9) 096008 doi: 10.1117/1.JBO.22.9.096008
Published in: Journal of Biomedical Optics Volume 22, Issue 9
Show Author Affiliations
Zhao Liu, The Univ. of Manchester (United Kingdom)
Juliana Gomez, Colgate-Palmolive Co. (United States)
Soniya Khan, Colgate-Palmolive Co. (United States)
Deborah Peru, Colgate-Palmolive Co. (United States)
Roger Ellwood, The Univ. of Manchester (United Kingdom)

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