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Journal of Biomedical Optics

System calibration method for Fourier ptychographic microscopy
Author(s): An Pan; Yan Zhang; Tianyu Zhao; Zhaojun Wang; Dan Dan; Ming Lei; Baoli Yao
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Paper Abstract

Fourier ptychographic microscopy (FPM) is a recently proposed computational imaging technique with both high-resolution and wide field of view. In current FPM imaging platforms, systematic error sources come from aberrations, light-emitting diode (LED) intensity fluctuation, parameter imperfections, and noise, all of which may severely corrupt the reconstruction results with similar artifacts. Therefore, it would be unlikely to distinguish the dominating error from these degraded reconstructions without any preknowledge. In addition, systematic error is generally a mixture of various error sources in the real situation, and it cannot be separated due to their mutual restriction and conversion. To this end, we report a system calibration procedure, termed SC-FPM, to calibrate the mixed systematic errors simultaneously from an overall perspective, based on the simulated annealing algorithm, the LED intensity correction method, the nonlinear regression process, and the adaptive step-size strategy, which involves the evaluation of an error metric at each iteration step, followed by the re-estimation of accurate parameters. The performance achieved both in simulations and experiments demonstrates that the proposed method outperforms other state-of-the-art algorithms. The reported system calibration scheme improves the robustness of FPM, relaxes the experiment conditions, and does not require any preknowledge, which makes the FPM more pragmatic.

Paper Details

Date Published: 12 September 2017
PDF: 11 pages
J. Biomed. Opt. 22(9) 096005 doi: 10.1117/1.JBO.22.9.096005
Published in: Journal of Biomedical Optics Volume 22, Issue 9
Show Author Affiliations
An Pan, Xi'an Institute of Optics and Precision Mechanics, CAS (China)
Univ. of Chinese Academy of Sciences (China)
Yan Zhang, Xi'an Institute of Optics and Precision Mechanics, CAS (China)
Univ. of Chinese Academy of Sciences (China)
Tianyu Zhao, Xi’an Institute of Optics and Precision Mechanics, CAS (China)
Univ. of Chinese Academy of Sciences (China)
Zhaojun Wang, Xi'an Institute of Optics and Precision Mechanics, CAS (China)
Univ. of Chinese Academy of Sciences (China)
Dan Dan, Xi'an Institute of Optics and Precision Mechanics, CAS (China)
Univ. of Chinese Academy of Sciences (China)
Ming Lei, Xi'an Institute of Optics and Precision Mechanics, CAS (China)
Baoli Yao, Xi'an Institute of Optics and Precision Mechanics, CAS (China)


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