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Journal of Biomedical Optics • Open Access

Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser
Author(s): Marco Andreana; Tuan Le; Anders K. Hansen; Aart J. Verhoef; Ole B. Jensen; Peter E. Andersen; Paul Slezak; Wolfgang Drexler; Alma Fernández; Angelika Unterhuber

Paper Abstract

We have developed an epi-detected multimodal nonlinear optical microscopy platform based on a compact and cost-effective laser source featuring simultaneous acquisition of signals arising from hyperspectral coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence, and second harmonic generation. The laser source is based on an approach using a frequency-doubled distributed Bragg reflector-tapered diode laser to pump a femtosecond Ti:sapphire laser. The operational parameters of the laser source are set to the optimum trade-off between the spectral and temporal requirements for these three modalities, achieving sufficient spectral resolution for CARS in the lipid region. The experimental results on a biological tissue reveal that the combination of the epi-detection scheme and the use of a compact diode-pumped femtosecond solid-state laser in the nonlinear optical microscope is promising for biomedical applications in a clinical environment.

Paper Details

Date Published: 17 August 2017
PDF: 7 pages
J. Biomed. Opt. 22(9) 091517 doi: 10.1117/1.JBO.22.9.091517
Published in: Journal of Biomedical Optics Volume 22, Issue 9
Show Author Affiliations
Marco Andreana, Medizinische Univ. Wien (Austria)
Tuan Le, FEMTOLASERS Produktions GmbH (Austria)
Anders K. Hansen, Technical Univ. of Denmark (Denmark)
Norlase ApS (Denmark)
Aart J. Verhoef, Medizinische Univ. Wien (Austria)
Technische Univ. Wien (Austria)
Ole B. Jensen, DTU Fotonik (Denmark)
Peter E. Andersen, Technical Univ. of Denmark (Denmark)
Paul Slezak, Ludwig Boltzmann Institut (Austria)
Wolfgang Drexler, Medizinische Univ. Wien (Austria)
Alma Fernández, Medizinische Univ. Wien (Austria)
Technische Univ Wien. (Austria)
Angelika Unterhuber, Medizinische Univ. Wien (Austria)

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